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10 ng of the phrl-tk vector  (Promega)

 
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    Promega 10 ng of the phrl-tk vector
    10 Ng Of The Phrl Tk Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10 ng of the phrl-tk vector/product/Promega
    Average 90 stars, based on 1 article reviews
    10 ng of the phrl-tk vector - by Bioz Stars, 2026-04
    90/100 stars

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    Effect of PLE0 on the activation of NF-κB and AP-1 in HT-29 cells. ( A , C ) The NF-κB-Luc and AP-1-Luc reporter vectors were transiently transfected into cells, and an internal control was established using the phRL-TK vector. After applying a prespecified concentration of PLE0 for 24 h treatment, the cells were collected. The assessment of luciferase activity was evaluated utilizing the luciferase assay kit provided by Promega. ( B , D ) Total cellular proteins were detected using western blot analysis with specific antibodies. The means ± SD of three separate experiments were reported. The differences between groups were assessed using analysis of variance and Dunnett’s post hoc test to determine significance. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.

    Journal: Polymers

    Article Title: Anti-Metastatic Effects of Standardized Polysaccharide Fraction from Diospyros kaki Leaves via GSK3β/β-Catenin and JNK Inactivation in Human Colon Cancer Cells

    doi: 10.3390/polym16091275

    Figure Lengend Snippet: Effect of PLE0 on the activation of NF-κB and AP-1 in HT-29 cells. ( A , C ) The NF-κB-Luc and AP-1-Luc reporter vectors were transiently transfected into cells, and an internal control was established using the phRL-TK vector. After applying a prespecified concentration of PLE0 for 24 h treatment, the cells were collected. The assessment of luciferase activity was evaluated utilizing the luciferase assay kit provided by Promega. ( B , D ) Total cellular proteins were detected using western blot analysis with specific antibodies. The means ± SD of three separate experiments were reported. The differences between groups were assessed using analysis of variance and Dunnett’s post hoc test to determine significance. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.

    Article Snippet: pNF-κB-Luc or pAP-1-Luc reporter vector along with the phRL-TK vector (Promega; Madison, WI, USA) using Lipofectamine LTX™ (Thermo Fisher Scientific; Waltham, MA, USA) was transfected into HT-29 and HCT-116 cells for 18 h. The luciferase activity of each cell was estimated using the luciferase assay (Promega; Madison, WI, USA).

    Techniques: Activation Assay, Transfection, Plasmid Preparation, Concentration Assay, Luciferase, Activity Assay, Western Blot